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. 2021 Jun 10;7:137. doi: 10.1038/s41420-021-00496-y

Fig. 2. Fadraciclib downregulates MCL-1, resulting in rapid induction of apoptosis in lines treated with 1 µM of fadraciclib for 4 and 24 h. SH-PTP2 was used as an internal AML cell lines.

Fig. 2

A Representative Western blot of OCI-AML3, MOLM-13, and MV4-11 cell protein loading control. B Densitometric analysis of serine 807/serine 811 (S807/S811) phosphorylated and total Rb. C Densitometric analysis of serine 2 (S2) phosphorylated RNAPII and total RNAPII. D Densitometric analysis of MCL-1. E Densitometric analysis of cleaved poly (ADP-ribose) polymerase-1 (PARP1). F Heatmap demonstrating fold changes relative to NDC of gene expression of OCI-AML3, MOLM-13, and MV4-11 cell lines treated with 0.75, 0.5, and 1 µM of fadraciclib, respectively, for 4 and 24 h. G Fold changes of CDK9, MCL1, PPP1R10, and E2F1 gene expression as compared with NDC. n = 3. Graphs depict mean ± SD (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). Data were compared using the Student’s t-test.