Alignment of the deduced amino acid sequences of the S1 subunit of the S1 protein of IBV strains CK/CH/SCMY/160315, LDT3-A, 140913, GX-NN-13, LZ05, GX-NN11033, 111119 and NN1111. Amino acid sequences encoding the 3 hypervariable regions are shown in bold. The yellow box represents differential sites compared with other GI-28 types, while missing sites are demarcated as a red box. Green indicates unique mutation sites, and * represents differential site compared with the LDT3-A strain.