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Absolute quantitative RT-qPCR was used to determine viral RNA load level of the trachea, kidney, lung, bursa of Fabricius, cecal tonsil, spleen, and liver. Levels between the challenge group and the negative group were statistically significant at 4, 8, and 12. Asterisk (*) denotes the significant difference from the control group, with significance indicators corresponding to: (∗) significant, P< 0.05; (∗∗) highly significant, P< 0.01; (∗∗∗) very highly significant, P< 0.001.
