Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 10;11:12298. doi: 10.1038/s41598-021-91804-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

MicroRNA profiling of serum EVs obtained from the patients with gallbladder cancer and benign gallbladder disease, and healthy controls. (a) Serum EVs eluted in fraction #4 are observed as particles of 40–150 nm in size by transmission electron microscopy in all groups (× 25,000). Representative images of each group are presented. Scale bars represent 100 nm. GBC, gallbladder cancer; Benign, benign gallbladder disease; HCs, healthy controls. (b) The median size of isolated EVs measured by Zetasizer was 72 nm (range: 40–150). A representative image is shown. (c) The median protein concentration in #4 fraction was 46.6 μg/ml (IQR: 12.3–73.6) in the GBC (N = 50), and was 33.7 μg/ml (IQR: 19.4–71.0) in the Benign and the HCs (N = 69) (not significant). (d) The numbers of detected miRNAs in serum EVs isolated from the GBC and the combination of the Benign and the HCs is shown in a Venn diagram. (e) The fold change in the expression of each common 1240 miRNA in serum EVs in the GBC relative to the Benign and the HCs. (f) Thirty-nine miRNAs in serum EVs in the GBC showed a > twofold change relative to the Benign and the HCs.