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. 2021 Jun 10;4:713. doi: 10.1038/s42003-021-02251-6

Fig. 5. Earlier commitment to memory T cells after immunization with lentiviral vector compared to Ad5 vector.

Fig. 5

C57BL/6 mice (n = 10/group) were immunized i.m with 1 × 107 IGU of Ad5-CMV-EsxH or 5 × 107 TU of LV-β2m-EsxH. Various profiles of functional antigen-specific CD8+ T subsets were identified and assessed by cytometry at 28 and 90 dpi by use of PE EsxH TET, used together with antibodies specific to CD62L, CD44, CD127, and KLRG1 markers. a Gating strategy to distinguish TCM (central memory cells), TEM (effector memory cells), short-lived effector cells (SLECs), memory precursor cells (MPECs), early effector cells (EECs) and CD127+ KLRG1+ double-positive (DP) cells. b Violin plots showing the distribution of TEM and TCM at 28 or 90 dpi. c Composition of SLECs, MPECs, EECs, and DP within TEM subsets at 28 or 90 dpi. d Percentages of SLECs, MPECs, EECs, and DP, as determined at 28 or 90 dpi. For (b) and (d), statistical significance was determined via Two-way ANOVA with Tukey’s multiple comparison test (****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05). e Percentages of SLECs, MPECs, EECs, and DP at 28 or 90 dpi after immunization i.m with 1 × 107 IGU/mouse of Ad5-CMV-OVA or 5 × 107 TU/mouse of LV-β2m-OVA. Statistical significance was determined via Mann–Whitney t-test (**p < 0.01, *p < 0.05). For (b), (d), and (e), the violin plots show the median (black line) and the interquartile range (dashed line). Each dot represents biological replicate. Statistical test value P < 0.05 is not indicated on the graphs.