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. 2021 Jun 10;12:3528. doi: 10.1038/s41467-021-23793-7

Fig. 3. In vitro cytotoxicity.

Fig. 3

a–d Cell killing potency in the breast cancer cell lines KPL-4 (a), JIMT-1 (b), MDA-MB-231 (c), and JIMT-1(MDR1+) (d). Unconjugated N297A trastuzumab (black circle), MMAF DAR 2 ADC (red square), MMAE DAR 2 ADC (light green triangle), MMAF DAR 4 ADC (magenta square), MMAE DAR 4 ADC (green triangle), MMAE DAR 6 ADC (black open square), MMAE/F 2 + 2 ADC (dark purple inversed triangle), MMAE/F 2 + 4 ADC (light purple diamond), and MMAE/F 4 + 2 ADC (cyan square). Concentrations are based on the antibody dose without normalizing to each DAR. All assays were performed in quadruplicate. Data are presented as mean values ± SEM (n = 3 for N297A trastuzumab, MMAF DAR 4 ADC, and MMAE DAR 6 ADC in KPL-4, and MMAE DAR 2 and MMAE DAR 2 ADCs in JIMT-1; n = 4 for other groups). Source data are available as a Source Data file.