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. 2021 Jun 10;12:3528. doi: 10.1038/s41467-021-23793-7

Fig. 7. Evaluation of payload delivery efficiency by the dual conjugate format.

Fig. 7

a Structures of anti-HER2 AF488/Cy5 dual-dye conjugate (DOL of 2 + 2) and single-dye variants (DOL of 2). b, c Whole tumor fluorescence imaging of JIMT-1/MDA-MB-231 (4 : 1) admixed tumors 24 h after intravenous administration of mAb–AF488/Cy5 (3 mg kg−1, black circle) or a 1 : 1 cocktail of mAb–AF488 and mAb–Cy5 (3 mg kg−1 each, black square) into tumor-bearing female NU/J mice (n = 3 per group). Fluorescence images of the whole tumors (b) and semi-quantification (c) detected using a 700 nm channel. df Fluorescence microscopic imaging of the tumor tissues. Fluorescence images of the frozen sections (AF488 and Cy5 channel, scale bar: 300 µm (d) and semi-quantification (AF488 channel (e) and Cy5 channel (f)). For tissue section analysis, six areas were randomly selected from the tissue sections and normalized signal intensity (intensity in each region of interest divided by area) was calculated using ImageJ software. Data are presented as mean values ± SEM (n = 6). For statistical analysis, a two-tailed Welch’s t-test was used. This experiment was repeated twice independently with similar results. Source data are available as a Source Data file. AF488, Alexa Fluor® 488; DOL, degree of labeling.