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. 2021 Jun 10;12:3547. doi: 10.1038/s41467-021-23828-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Shows the m/z value distribution of individual experiments analyzed with the Thermo Orbitrap Fusion Lumos. Run 10 (green), Run 11(blue), and Run 12 (purple) are compared with all ions appearing in total in all samples after thresholding. The graph on the right (b) shows the online analytical result on the Advion L-CMS series of Run 10 and Run 11. The online analytical system failed at Run 12, which is the reason that there is not a third comparable dataset. c It shows a comparison of the offline analytical data of all three runs. The measurements of run 11 and 12 were performed at the same time, while run 10 was measured at a later date. This means that the differences between run 10 and the other runs may be partially due to a difference in performance.