REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse anti-human CD3-BV510 (clone UCHT1)	Biolegend	Cat# 300448, RRID: AB_2563468
Mouse anti-human CD19-APC-Cy7 (clone HIB19)	Biolegend	Cat# 302218, RRID: AB_314248
Mouse anti-human CD21-PE-Cy7 (clone Bu32)	Biolegend	Cat# 354912, RRID: AB_2561577
Mouse anti-human CD27-BV605 (clone O323)	Biolegend	Cat# 302830, RRID: AB_2561450
Mouse anti-human IgM-BV785 (clone MHM-88)	Biolegend	Cat# 314544, RRID: AB_2800832
Human anti-human IgA-VioBright 515 (clone REA1014)	Miltenyi Biotec	Cat# 130-116-886, RRID: AB_2727744
Mouse anti-human IgG-BV421 (clone G18-145)	BD	Cat# 562581, RRID: AB_2737665
Mouse anti-human CD3-APC-Cy7 (clone UCHT1)	Biolegend	Cat# 300426, RRID: AB_830755
Mouse anti-human CD4-BV786 (clone SK3)	BD	Cat# 563877, RRID: AB_2738462
Mouse anti-human CD8-BV510 (clone RPA-T8)	Biolegend	Cat# 301048, RRID: AB_2561942
Mouse anti-human CD45RO-BV605 (clone UCHL1)	Biolegend	Cat# 304238, RRID: AB_2562153
Mouse anti-human CD40L-PE-CF594 (clone TRAP1)	BD	Cat# 563589, RRID: AB_2738297
Mouse anti-human CD279-PE-Cy7 (clone EH12.2H7)	Biolegend	Cat# 329918, RRID: AB_2159324
Mouse anti-human CXCR5-FITC (clone J252D4)	Biolegend	Cat# 356912, RRID: AB_2561894
Mouse anti-human IFNγ-PE (clone B27)	Biolegend	Cat# 506507, RRID: AB_315440
Mouse anti-human IL4-BV421 (clone MP4-25D2)	Biolegend	Cat# 500826, RRID: AB_2561679
Mouse anti-human IL13-BV421 (clone JES10-5A2)	Biolegend	Cat# 501916, RRID: AB_2616748
Mouse anti-human AF647 (clone 3A3-N2)	Biolegend	Cat# 513006, RRID: AB_1227661
Bacterial and virus strains
SARS-CoV-2 (Wuhan strain)	Isolated from patient	N/A
Biological samples
Peripheral blood of Covid-19-recovered individuals	Heinsberg county, NRW, Germany	N/A
Peripheral blood of Covid-19-severely ill individuals	University Hospital Bonn, Community Hospital Heinsberg, Germany	N/A
Chemicals, peptides, and recombinant proteins
APC Streptavidin	Biolegend	Cat# 405207
PE Streptavidin	Biolegend	Cat# 405204
Biotinylated SARS-CoV-2 (COVID-19) S1 protein, His, Avitag (MALS verified)	BioCat	Cat# S1N-C82E8-200ug-AC
DPBS, no calcium, no magnesium	ThermoFisher Scientific	Cat# 14190094
RPMI 1640 Medium	ThermoFisher Scientific	Cat# 21875034
DMEM, high glucose	ThermoFisher Scientific	Cat# 41965039
MEM Eagle	Pan Biotech	Cat# P03-2710
Sodium azide	Sigma-Aldrich	Cat# S2002-25G, CAS: 26628-22-8
EDTA (0.5 M), pH 8.0, RNase-free	ThermoFisher Scientific	Cat# AM9260G
Penicillin-Streptomycin (5,000 U/mL)	ThermoFisher Scientific	Cat# 15070063
L-Glutamine (200 mM)	ThermoFisher Scientific	Cat# 25030081
BD GolgiStop	BD	Cat# 554724
BD GolgiPlug	BD	Cat# 555029
Ionomycin calcium salt	Sigma-Aldrich	Cat# I3909-1ML
PMA	Sigma-Aldrich	Cat# P1585-1MG, CAS: 16561-29-8
Dimethyl sulphoxide (DMSO)	Carl Roth	Cat# A994.2
Pancoll human, Density: 1.077 g/ml	Pan Biotech	Cat# P04-60500
Sera Plus, EU approved regions, special processed FBS	Pan Biotech	Cat# P30-3702
Carboxymethylcellulose sodium salt	Sigma-Aldrich	Cat# C5013-1KG
OptiPRO SFM	ThermoFisher Scientific	Cat# 12309019
Crystal violet	Carl Roth	Cat# T123.3
Ethanol	Carl Roth	Cat# T171.4
Formaldehyde solution 37%	Carl Roth	Cat# CP10.2
HEPES solution	Sigma-Aldrich	Cat# H0887-20ML
Rainbow Fluorescent Particles	Biolegend	Cat# 422905
PepTivator SARS-CoV-2 Prot_S	Miltenyi Biotec	Cat# 130-126-700
PepTivator SARS-CoV-2 Prot_M	Miltenyi Biotec	Cat# 130-126-702
PepTivator SARS-CoV-2 Prot_N	Miltenyi Biotec	Cat# 130-126-698
Critical commercial assays
Zombie Aqua Fixable Viability Kit	Biolegend	Cat# 423102
FcR Blocking Reagent, human	Miltenyi Biotec	Cat# 130-059-901
CD19 MultiSort Kit, human	Miltenyi Biotec	Cat# 130-055-301
Anti-SARS-CoV-2-ELISA (IgG)	Euroimmun	EI 2606-9601G
Anti-SARS-CoV-2-ELISA (IgA)	Euroimmun	EI 2606-9601A
Fixation/Permeabilization Solution Kit	BD	Cat# 554714
Experimental models: Cell lines
VERO C1008 (Vero 76, clone E6, Vero E6)	ATCC	Cat# CRL-1586
Software and algorithms
FlowJo Software	TreeStar	Version 10.0.7
Prism	GraphPad	Version 9
SPICE	NIH	Version 6.1