Fig. 2.

3-Dimensional printing using Polylactic Acid and infill design allows for bacterial growth within 24h of sterilization. PLA-based 3D-printed cylinders with infill were incubated for 24 and 72 h in sterile 1-Lieter beakers and 125mL sterile LB broth following sanitization using 200mL isopropanol (91%) for 20 min. A) Representative image of 24 h post sanitization, showing robust bacterial growth. B) Gram staining revealed both gram-positive cocci and gram-negative rods. C) OD600 was determined to quantify the relative bacterial growth pre- and post-sanitization. Significant bacterial growth was observed at both 24-h and 72-h timepoint. * p < 0.05; ** p < 0.01 when compared to LB broth control