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. 2021 May 28;12:693777. doi: 10.3389/fphar.2021.693777

FIGURE 4.

FIGURE 4

ZJS decreased the number of osteoclasts and the expression of osteoclast-related proteins in the ankle joints of CIA mice. (A) Representative immunohistochemical staining images of TRAP, cathepsin K, OPG, and RANKL in the ankle joints of mice in the indicated groups (scales bars = 100 μm). (B) The quantification of cathepsin K, RANKL, and OPG expression in the ankle joints of mice in the indicated groups (n = 3). (C) The number of TRAP-positive cells in the ankle joints in the indicated groups (n = 5). (D) The content of RANKL in the serum of mice in the indicated groups (n = 5). (E) Ratio of the IHC intensity of OPG and RANKL in the ankle joints in the indicated groups (n = 3). Values are presented as the mean ± SEM. ns: no significance. ## p < 0.01, ### p < 0.005, #### p < 0.001, compared with the normal group. *p < 0.05, **p < 0.01, ****p < 0.001, compared with the vehicle group (One-way ANOVA test).