Figure 4.

Effects of dracorhodin perchlorate on the protein expression levels of β-catenin, AKT, ERK and p38 proteins in HaCaT keratinocytes. Cells were incubated with 0, 1 and 2 µg/ml dracorhodin perchlorate for 12 h. Whole cell lysates were prepared before the levels of cell migration-related proteins were analyzed by western blot analysis. The protein levels of (A) β-catenin, (B) p-AKT, AKT, p-ERK, ERK, p-p38 and p38 were detected. All blots were normalized to β-actin to ensure equal loading.