Fig. 7.

Concentration, absolute fluxes and volume specific fluxes for glucose, lactate and glutamine. Concentration in [mM], absolute fluxes in [mM/h], volume specific fluxes in [mmol/gDW/cell] and time scale in hours. The data for the control cultures are shown in pink (37°C‐1) and red (37°C‐2), and the temperature-shifted cultures in cyan (32°C‐1) and blue (32°C‐2).The glucose and glutamine consumption fluxes were higher for the control cultures, whereas they were more stable over time for the temperature-shifted cultures. Similarly, the lactate flux was higher for the control cultures until 168 h. At stationary phase cells began to consume lactate. Differences in fluxes between the control and temperature-shifted cultures were reduced when fluxes were expressed as specific fluxes. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)