Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 11;17(8):1979–1994. doi: 10.7150/ijbs.58422

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The author(s)

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.

PMC Copyright notice

SH3PXD2A-AS1 maintains CRC stem cell properties and promotes cell angiogenesis. (A) Relative expression of SH3PXD2A-AS1 in adherent CRC cells and the corresponding suspension cultured tumor-spheres. Values were normalized against 18s rRNA from three independent experiments. (B-C) Effect of SH3PXD2A-AS1 overexpression or knockdown on mammosphere formation detected by mammosphere formation assays were quantified by sphere numbers. (D) Western blot of CSC markers: Nanog, OCT4, CD133 and CD44 in SH3PXD2A-AS1 knockdown SW620 cell. GAPDH was used as a loading control. (E-F) SH3PXD2A-AS1 positively regulated tube formation in CRC cells. The numbers of tubular structures formed by HUVECs were counted for in HCT116 and SW620 cells with SH3PXD2A-AS1 overexpression or knockdown. Data are presented as the means ± SD for experiments in triplicate. (G) Detection of HIF1α and VEGF protein expression in HCT116 cells with SH3PXD2A-AS1 overexpression. GAPDH was used as a loading control. Statistical analysis was performed by using two-tailed Student's. *p < 0.05, ***p < 0.001.