Figure 6.

SH3PXD2A-AS1 promotes tumor growth and metastasis in vivo. (A) Stable SH3PXD2A-AS1 knockdown and control SW620 cells were subcutaneously injected into BALB/c female nude mice (n = 9, for each experimental group), and 2 mg/ml of Dox administered in 5% drinking water was used to induce shRNA expression. 1 × 10⁶ cells and Matrigel (Corning; 1:1 ratio) were subcutaneously injected into each mouse. Three weeks later, the xenograft tumors were peeled off and photographed. (B-C) Effect of SH3PXD2A-AS1 knockdown on the xenograft model was assessed by evaluating tumor volume and tumor weight. ***P<0.001 (D) SH3PXD2A-AS1 overexpression HCT116 cells were injected via the lateral tail veins. Representative lung images at week 8, corresponding hematoxylin-eosin-stained lung sections are shown. (E) Lung nodules were analyzed as the numbers of nodules per mouse. Statistical analysis was performed by using two-tailed Student's t test. ***P<0.001. (F) A cartoon summarizing our findings. SH3PXD2A-AS1 interacts with p53 protein and regulated p53 mediated gene transcription, thereby promoting CRC growth, and metastasis.