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. 2021 May 11;17(8):1995–2008. doi: 10.7150/ijbs.59019

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Rescue with NEK2 promoted cancer progression. (A-B) The clone numbers of A549 and H1299 cells transfected with non-specific control siRNAs (NC), NEK2-specific siRNAs (siRNA) and NEK2-specific siRNAs with NEK2 overexpression plasmids (siRNA+OE) in colony formation assays. (C) CCK-8 assays in A549 and H1299 cells transfected with non-specific control siRNAs (NC), NEK2-specific siRNAs (siRNA) and NEK2-specific siRNAs with NEK2 overexpression plasmids (siRNA+OE). (D) Immunofluorescence of Ki67 in A549 and H1299 cells transfected with non-specific control siRNAs (NC), NEK2-specific siRNAs (siRNA) and NEK2-specific siRNAs with NEK2 overexpression plasmids (siRNA+OE). Data are presented as mean ± SD of 3 independent experiments. *P < 0.05.

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