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. 2021 May 5;17(8):1895–1908. doi: 10.7150/ijbs.59855

Figure 4.

Figure 4

Compound 3K reduced colony formation and cell proliferation and induced the cell cycle arrest in SK-OV-3 cells. (A) Effect of compound 3K (1, 2.5, or 5 µM) on proliferation of SK-OV-3 cells following treatment for 48 h. Cell proliferation was checked with an IncuCyte software every 6 h. (B) Representative photographs and quantitative analysis of colony formation assay of SK-OV-3 cells treated with indicated compound 3K concentrations in 6-well plates. Colony areas were measured using image analyzer. (C) SK-OV-3 cells were treated with compound 3K (1, 2.5, or 5 µM) for 24 h. After treatment, the cells were stained with propidium iodide (PI) and analyzed using flow cytometry. (D) Bar graph indicating the different phases of cell cycle distributions of SK-OV-3 cells treated with 0.1% DMSO control or compound 3K. (E) Effect of compound 3K on the expression of cell cycle-related proteins. SK-OV-3 cells were treated for 24 h with compound 3K (1, 2.5, or 5 µM). The expression of cyclin B1, p-Cdc2, and Cdc2 were analyzed using western blot analysis. The values represent the mean ± SD. *p < 0.05 and **p < 0.01.