Skip to main content
. 2021 May 5;17(8):1895–1908. doi: 10.7150/ijbs.59855

Figure 8.

Figure 8

Compound 3K altered glycolytic function in SK-OV-3 cells. (A) The level of ECAR was measured using XF Glycolytic Stress Test kit. Following incubation for 24 h, compound 3K (1, 2.5, or 5 µM) was added to the plates followed by treatment with 15 mM glucose, 2 µM oligomycin, and 50 mM 2-DG subsequently. (B) Quantification of glycolytic function in SK-OV-3 cells following treatment. (C) Quantitative level of lactate in the culture media of compound 3K-treated cells. (D) Western blot analysis was performed to determine the protein expression levels of GLUT-1, LDHA, and MCT-4. The values represent the mean ± SD. *p < 0.05 and **p < 0.01.