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. 2021 May 11;17(8):1963–1978. doi: 10.7150/ijbs.55557

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EPB41L4A-AS2 directly binding to YBX1. (A) FISH assays were applied to determine the subcellular localization of EPB41L4A-AS2 in NPC. U6 was mainly located in the nucleus, while 18S was mainly located in the cytoplasm. (B) Nuclear mass separation assays demonstrated that EPB41L4A-AS2 was located both in the nucleus and cytoplasm. (C).SDS-PAGE gel was used to explore the EPB41L4A-AS2 specific binding protein gels (D) YBX1 was identified by SDS-PAGE. (E) RIP assays confirmed that EPB41L4A-AS2 directly binds to YBX1. (F) Subcellular location of YBX1 in SUNE1 cells. *P < 0.05 and **P < 0.01.