Figure 8.

PPD does not cause bone loss with a good safety. (A) After 28 days for treatment, the bodyweight of the mice was recorded. (B) The H&E staining of the kidneys and livers, and the TRAP staining and ALP staining on mice femurs. (C) The serum biochemical index, and the levels of TRAP and ALP in the serum had been detected (n=6-9). (D) Three-dimensional micro-CT images of the trabecular microstructure of distal tibia metaphysis at the transaxial and axial view. (E) The BMD, BV, TV, BV/TV, trabecular thickness, and trabecular number of EMs mice in the control group, the PPD group and the GnRHa group were detected by Micro-CT (n=10). Mean ± SEM, NS, no significant difference, *p<0.05, **p<0.01.