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. 2021 Jun 9;218(8):e20202489. doi: 10.1084/jem.20202489

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© 2021 Viant et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S4. — Somatic mutation in activated B cells. Related to Fig. 5. (A) Graphs show the level of expression of ACIDA in Fo B cells, activated B cells (Act B), GC cells, IgM, or switch isotype–expressing memory B cells (MBC) obtained by RNA-seq. (B) Representative flow cytometry profiles showing B220⁺ B cells 3 d after HIV-1 TM4-Core immunization and tamoxifen (TAM) administration and Act B (GL7⁺, CD38⁺, ZSGreen⁺, IgD⁺, CD95⁺) sorted from the same sample. (C) Graph showing the number of somatic mutations (nucleotides, IgH + IgL [H+L nt]) in the antibodies obtained from Act B 3 d after immunization (three independent experiments, n = 3–5 mice per group, each dot represents one antibody, n = 84 antibody genes sequenced).