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. 2021 Jun 9;220(8):e202102043. doi: 10.1083/jcb.202102043

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© 2021 Galloni et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 7. — Arp3B Met293 is oxidized by MICAL2. (A) Immunoblot analysis of GFP-Trap pulldowns from HeLa cells lysates stably expressing GFP or the indicated GFP-tagged proteins demonstrates that Arp3B (red), but not Arp3 (blue), is oxidized (*) in a Met293-dependent fashion. (B) RT-PCR analysis of the level of MICAL2 mRNA in HeLa cells stably expressing GFP or GFP-Arp3B and treated with MICAL2 siRNA. Error bars represent SEM from three independent experiments. The immunoblot of GFP-Trap pulldown assays on cells stably expressing GFP-Arp3B shows the level of Arp3B Met293 oxidation after treatment with MICAL2 siRNA.