Figure S4.

Coronin 1C and MICAL2 cooperate to promote actin disassembly. (A) RT-PCR analysis of the level of MICAL2 and coronin 1C mRNA in HeLa cells stably expressing GFP^PA-tagged Arp3 and Arp3B and treated with MICAL2 and/or coronin 1C (Coro1C) siRNA. (B) Quantification of the half-life of photoactivated GFP^PA-tagged Arp3 or Arp3B in actin tails in cells treated with coronin 1C and/or MICAL2 siRNA. (C) RT-PCR analysis of the level of MICAL2, Arp3B, and coronin 1C mRNA in HeLa cells stably expressing Cherry-GFP^PA-β-actin and treated with different combinations of MICAL2, Arp3B, and coronin 1C siRNA. (D) Quantification of the half-life of photoactivated Cherry-GFP^PA-β-actin in actin tails in cells treated with different combinations of Arp3B, coronin 1C, and MICAL2 siRNA. The graphs in B and D represent the best-fitting curve for each condition (continuous line), together with the average normalized intensity of the GFP signal at every time point (error bars represent the SEM for the indicated number of tails). Error bars for RT-PCR analysis represent SEM from three independent experiments.