Figure 2.

Plt-mito transplantation prevents HR-induced mitochondrial malfunction and mitochondria-mediated apoptosis. Plt-mito were cocultured with the cells under the normoxic and HR condition. To inhibit FUNDC2-evoked PIP3/Akt pathway, LY294002 was applied at the onset of plt-mito transplantation. To block the extra-mitochondrial terminals of FUNDC2, plt-mito were treated with antibodies to N- or C-term of FUNDC2 prior to transplantation. (A) ΔΨm of the recipient cells was assessed by JC-1 assay and observed by fluorescent microscopy. Scale: 500 nm. n =5. *P < .05 vs. control. Fluorescence changes from red to green as ΔΨm decreases. (B-E) Mitochondrial ATP, ROS, Cyto C leakage, and cell viability were analyzed as specified in the Methods, n = 5. *P < .05 vs. control.