Figure 2.

CircVAPA knockdown inhibits the proliferation, migration and invasion of oral cancer cells. a: Two siRNA targeting circVAPA (si-circVAPA#1 and si-circVAPA#2) were designed to knock down circVAPA expression. A negative control (NC) siRNA was also used. The Cal-27 cell line with the highest expression of circVAPA was selected for silencing experiments. The knockdown efficiency was detected by RT-qPCR. ***P < 0.001 compared with si-NC. b: The cell counting kit-8 method was used to detect the optical density (OD) values of Cal-27 cells in different groups (si-NC, si-circVAPA#1, si-circVAPA#2) at 450 nm at 0, 24, 48 and 72 hours. ***P < 0.001. c: The clone formation ability of Cal-27 cells in different groups was detected and quantified. ***P < 0.001 compared with si-NC. d: Transwell assay (without Matrigel) was used to detect the migration ability of Cal-27 cells in different groups. ***P < 0.001 compared with si-NC. e: Transwell assay (with Matrigel) was used to detect the invasion ability of Cal-27 cells in different groups. ***P < 0.001 compared with si-NC