Figure 5.

circVAPA regulates homeobox A7 (HOXA7) protein expression by targeting miR-132. a: The online StarBase database predicted a miR-132 binding site in the 3ʹ-untranslated region (UTR) of wildtype (WT) circVAPA. This site was then mutated (MUT). Luciferase reporter experiments were carried out in Cal-27 cells transfected with a negative control (miR-NC) or miR-132. ***P < 0.001 compared with miR-NC. b: The protein expression of HOXA7 in Cal-27 cells overexpressing miR-132 was detected by western blot. ***P < 0.001 compared with miR-NC. Glycerol 3-phosphate dehydrogenase (GAPDH) was used as an internal control. c: HOXA7 protein expression level in different groups of Cal-27 cells was detected by western blot. **P < 0.01 and ***P < 0.001. GAPDH was used as an internal control