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. Author manuscript; available in PMC: 2021 Jun 11.
Published in final edited form as: Biochemistry. 2020 May 20;59(21):1981–2002. doi: 10.1021/acs.biochem.0c00274

Figure 4.

Figure 4.

HN reduced Aβ aggregation, an effect reversed upon the addition of IGFBP-3. Pretreated monomeric Aβ (2 μM) was incubated with 2 μM HN in the absence or presence of 2 or 6 μM IGFBP-3 ((A) Aβ40, AS-72215 and (B) Aβ42, AS-72216). Thioflavin fluorescence (Ex 440 nm, Em 484 nm) was monitored at 37 °C for 335 min every 2.5 min, with 15 s of shaking between the readings. Assay buffer alone, IGFBP-3, or HN was used as a blank. Each measurement was corrected for the baseline fluorescence in the absence of Aβ. The change in fluorescence units (RFU) relative to the first measurement at t0 is shown. The data were normalized to the maximum ThT level and plotted using GraphPad Prism 8.3.1.