Figure 6.

Both AChE and HN were found in a complex with Aβ from the medium of A549 cells using 6E10 antibodies; however, the addition of an exogenous HN peptide reduced but did not abolish the binding of AChE to Aβ. Anti-Aβ specific antibodies (6E10) were added (1:1000 dilution) to the ELISA wells. The wells were blocked, and 300 μL of the conditioned medium (0.5 μg/μL of A549 cells or H1299 cells, 72 h after serum starvation) was added. The amount of HN and AChE bound was detected using the corresponding specific primary antibodies and then processed as described in the Experimental Procedures. The fold change relative to the controls incubated with all components except the primary antibodies was calculated and fit with a nonlinear regression curve using GraphPad Prism 8.3.1. The data represent the mean ± SD of three separate experiments, each of which was performed in triplicate.