FIGURE 6.

Effect of LPS and TNFα on cell migration in the CE-OOC co-culture condition. A, Fluorescence microscopy of co-culture experiments showed that both ectocervical epithelial cells (blue) and endocervical epithelial cells (yellow) migrate through the collagen-filled microchannels. The bottom panel highlights the migrated ectocervical epithelial cells (blue) and endocervical epithelial cells (yellow) that have integrated into the microchannels. Scale bar, 200 μm. The right panel is a schematic representing the migration of cervical epithelial cells inside the microchannels. B, Quantification of migrating ectocervical epithelial cells that enter through collagen-filled microchannels. n = 3 biological replicates. C, Quantification of migrating endocervical epithelial cells that enter through the collagen-filled microchannels. Error bars represent mean ± SEM. n = 3 biological replicates