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. 2021 Jun 11;21:268. doi: 10.1186/s12870-021-03045-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — The genes involved in abiotic response. a Gene ontology enrichment analysis of DEGs between brs1-1D and WS2 (q-value < 0.01). The black and grey dots represent GOs that are up-regulated and down-regulated in brs1-1D respectively. The terms enriched GOs are divided into five categories. Gene ratio indicates the number of DEGs from different term, divided by the total number of DEGs. b The qRT-PCR detection of cold related genes (CBF1, CBF2, CBF3, RD29A, COR413, KIN2), redox related gene (PER10) and ethylene synthesis related genes (ACS4, ACO3). These genes were significant up-regulated in brs1-1D compared to WS2, confirmed the results from RNA sequencing (Table S1). All results are presented as mean ± SD from three biological replicates at least. The asterisks indicate a statistically significant difference (Student’s t-test, **p < 0.01, *p < 0.05)