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. 2021 Jun 11;21:268. doi: 10.1186/s12870-021-03045-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — The brs1-1D shows a higher cold sensitivity. The qRT-PCR was performed in each genotype with and without freeze treatment (4 ℃ for 3 h). The columns represent the fold change of gene expression after freeze treatment compared to untreated control. All results are shown as mean ± SD from three biological replicates at least. The asterisks indicate a statistically significant difference (Student’s t -test, *p < 0.05). a Cold signaling genes responses with freeze treatment. b Stress related genes response with freeze treatment. Including ethylene synthesis related genes (ACS4 and ACO3) and redox related gene PER10