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. 2021 Jun 11;21:268. doi: 10.1186/s12870-021-03045-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — The phenotypes of seedling in cold treatment. 7 DAG seedlings were shifted to -6 °C for 2, 3 and 4 h and then returned to normal conditions (22 °C) for 36 h. a The phenotypes of WS2, brs1-1 and brs1-1D grown on 1/2 MS medium under normal conditions and with cold treatment for 2 h, 3 h and 4 h. Scale bar = 2 cm. The black dots on the plate represent the growth position of the seedlings before the cold treatment. b The root elongation in WS2 and brs1-1D after a severe cold treatment. The root elongation was measured at 36 h after cold shock. Data is from three biological replicates. The same results were repeated at least two times. The asterisks indicate a statistically significant difference (Student’s t-test, ** p < 0.01)