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. 2021 Jun 10;12:342. doi: 10.1186/s13287-021-02421-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — LPS promotes colony formation and pluripotent marker expression in hepatocytes. a Left: Representative images of AML12 cells treated with or without LPS; right: quantification of colony diameters. Scale bars, 100 μm. b Left: Representative images of colony formation in AML12 cells after culture with or without LPS for 12 days; right: quantification of colony numbers. c The relative mRNA expression of pluripotent and stem cell markers in AML12 cells cultured in basic medium in the presence or absence of LPS for 24 h. mHPCs were used as a positive control. d Western blot assay for Sox2, cMyc, Klf4, and Sox9 in AML12 cells cultured in basic medium containing LPS for the indicated amounts of time. h: hours; d: days. **P < 0.01, ***P < 0.001