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. 2021 Jun 10;12:335. doi: 10.1186/s13287-021-02398-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — FoxA2 expression in the dopaminergic cell lineage of the SNpc. A Cell densities of the different subpopulations within the dopaminergic lineage (based on expression of TH and FoxA2) A in the SNpc of wild-type (WT) and “weaver” (wv) mice that received BNN-20 or saline (sal) from P14 to P60 and Β a characteristic example of immunostaining (with some of the TH+/ FoxA2− cells indicated by white arrows, and some of the TH+/FoxA2+ cells indicated by yellow arrows) [scale bar= 50 μm. a: decreased total TH+ cell densities (p < 0.05) compared to WT BNN-20 and WT sal groups (F = 7.94, p = 0.023 for genotype); b: increased TH+/FoxA2− densities (p < 0.05) compared to WT and wv sal groups (F = 25.971, p = 0.001 for drug), using two-way ANOVA followed by LSD post hoc analysis. n = 3 animals per group. Error bars are SEMs.]. C TH+/BrdU+ cell densities per optical field in the SNpc of the WT sal and WT BNN-20 groups, also showing the co-expression of FoxA2 [a: increased TH+/BrdU+/FoxA2+ and TH+/BrdU+/FoxA2− densities compared to WT sal; p < 0.05, using Student’s t test, n = 3 animals per group. Error bars are SEMs]. D Percentage of the newly described TH+/FoxA2− cells within the pools of total TH+ cells (in dark red), older (TH+/BrdU−, in blue), and newborn (TH+/BrdU+, in yellow) dopaminergic neurons in the WT SNpc post-administration of BNN-20 or saline (sal) (P14–P60) [a: increased % TH+/FoxA2− percentage compared to WT sal; p < 0.05, using Student’s t test, n = 3 animals per group. Error bars are SDs]. E A characteristic example of immunostaining indicating the 3 dopaminergic subpopulations of the SNpc: TH+/BrdU+/FoxA2+ (white arrow), TH+/BrdU−/FoxA2+ (cyan arrow), and TH+/BrdU−/FoxA2− (yellow arrow) [scale bar= 50 μm]