Fig. 6.

In vivo BNN-20 administration enhances differentiation of isolated NSCs into neurons, in primary cell cultures. A Histogram showing the average diameter of primary neurospheres generated from the SN of weaver mice having received saline or BNN-20 (P14–P60) [a: p < 0.05 compared to wv sal. Analysis was performed using Student’s t test, n = 5 optical fields per group. Error bars are SEMs.]. B Representative images after immunofluorescence staining for the NSPC markers Sox-2 (green) and nestin (red), as well as for the proliferation marker PCNA (white) [scale bars = 100 μm]. C Dot plot showing the percentage of SN-derived primary neurospheres containing at least one cell expressing the neuronal marker β-ΙΙΙ tubulin. Neurospheres were generated from SN of wild-type (WT) and “weaver” (wv) mice that had received BNN-20 or saline (sal) (P14–P60) [Error bars are SDs. a: p < 0.05 & a’: p < 0.01 compared to WT sal. b: p < 0.01 compared to WT BNN-20. c: p < 0.01 compared to wv sal. Analysis performed using two-way ANOVA followed by LSD post hoc test (p < 0.001, F = 24.207 for drug, p < 0.001, F = 245.576 for genotype, n = 5 optical fields per group)]. D Representative images after immunofluorescence staining for the NSPC marker Sox-2 (green) and the differentiation markers GFAP (white) and βIII tubulin (red) [scale bars= 100 μm]