Fig. 8.

No effect of BNN-20 on BrdU+ cells in the cortex and on astrogliogenesis. A Cell densities of total BrdU+ cells in the SNpc and cortex (CX) of wild-type (WT) animals after administration of BNN-20 or saline (sal) (P14–P60) [a < 0.01 compared to WT sal]. B Cell densities of total Sox2 expressing cells in the SNpc and CX WT animals after administration of BNN-20 or sal (P14–P60) [a < 0.01 compared to WT sal]. C Percentage of newborn (BrdU+/Sox2+) to total Sox2 expressing cells in the WT SNpc and the WT CX after administration of BNN-20 or saline (sal) (P14–P60) [a < 0.05 compared to WT sal]. D Cell densities of total GFAP+/Sox2+ expressing cells in the WT SNpc and the WT CX after administration of BNN-20 or saline (sal) (P14–P60) [a < 0.05 compared to WT sal]. Statistical analyses for the data presented in histograms A–D were performed using Student’s t test. n = 3 animals per group. Error bars are SEMs. E,F Representative immunofluorescence images of triple stainings against BrdU (green), GFAP (red), and Sox2 (white) in E the SNpc and F the CX of WT animals that received saline (sal) or BNN-20 (P14–P60) [scale bars= 50 μm]