FIGURE 3.

SIRT1 increment underlies Sal A-induced AMPK activation and UCP-1 upregulation. (A) Differentiated C3H10T1/2 adipocytes were treated with Sal A (80 μM) for 0, 2, 4 and 8 h. Intracellular SIRT1 contents were determined by Western blot. (B) After silencing SIRT1 by siRNA, cells were exposed to 80 μM Sal A for 4 h. The protein levels of UCP-1 and SIRT1 were detected by Western blot. (C) C3H10T1/2 adipocytes were transfected with siRNA for SIRT1 or AMPK, respectively. Then cells were treated with Sal A (80 μM) for 4 h. Total cellular lysates were collected for the immunoblotting assay for SIRT1, p-AMPK and AMPK. All values are denoted as means ± SD from three independent batches of cells. #p < 0.05 vs. the UT or Mock; *p < 0.05 vs. the Sal A treatment group. All groups contain two or three samples (n = 2 or n = 3).