FIGURE 5.

Sal A supplementation improves AMPK-SIRT1 pathway activation and prevents UCP-1 downregulation in eWAT of HFD-fed mice. (A) Results of the Western blot analysis of AMPK signaling in eWAT, including p-AMPK, and AMPK, SIRT1. (B) The gene expressions of UCP1, PGC-1α, and Prdm16, related to WAT browning. (C) The protein levels of UCP1 and quantification of the bands. The same internal reference (GAPHD) was used for p-AMPK, AMPK, SIRT1, and UCP1 quantitative analysis, since the results come from the same experiment based on the same batch of loading samples. In order to satisfy the logical rationality of the argument, we put the results in panel (A,C), respectively. #p < 0.05 vs. the ND group; *p < 0.05 vs. the HFD group. All groups contain 12 animals (n = 12).