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. 2021 May 28;13:673155. doi: 10.3389/fnagi.2021.673155

FIGURE 1.

FIGURE 1

Characterization of ChR2-EYFP expression in VGAT-ChR2(H134R)-EYFP transgenic mice. (A) Schematic representation of VGAT-ChR2-EYFP transgene (top) and an example of PCR gel electrophoresis (bottom). The first lane is the DNA size standard and the fragment size is given in base pairs on the left. The genotypes are described at the top of each lane. (B) The representative mechanically dissociated BF neuron with the illustrations of patch pipette and 470-nm LED light stimulation. (C) Confocal images of mouse BF slice show strong ChR2-EYFP expression in GABAergic neurons of VGAT-ChR2(H134R)-EYFP transgenic mice.