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. 2021 May 28;13:673155. doi: 10.3389/fnagi.2021.673155

FIGURE 4.

FIGURE 4

The effect of AMI (3 μM) on spontaneous IPSC frequency and amplitude in BF neurons from young and aged VGAT mice using the reduced synaptic preparation. Representative whole-cell voltage-clamp recordings (Vh = –60 mV) from a young (A) and aged (B) BF neuron in control (left) and 3 μM AMI (right). (C) Scatter plots with mean ± SEM of frequency (left panel, 1.05 ± 0.13 Hz for control, 0.24 ± 0.07 Hz in 3 μM AMI, paired t-test, *p < 0.05) and graph of normalized fold change in frequency (right panel, 0.24 ± 0.06 for 3 μM AMI, paired t-test, *p < 0.05) show that AMI decreases the frequency of sIPSCs in young BF neurons. (D) Scatter plots with mean ± SEM of frequency (left, control: 0.92 ± 0.28 Hz, 3 μM AMI: 0.18 ± 0.02 Hz, paired t-test, *p < 0.05) and graph of normalized fold change in frequency (right, 3 μM AMI: 0.35 ± 0.07, paired t-test, *p < 0.05) show that AMI decreases the frequency of sIPSCs in aged BF neurons. (E) Scatter plots with mean ± SEM of amplitude (left panel, 50.06 ± 6.62 pA in control, 31.37 ± 4.44 pA in 3 μM AMI, paired t-test, *p < 0.05) and graph of normalized fold change in amplitude (right panel, 0.65 ± 0.07 in 3 μM AMI, paired t-test, *p < 0.05) show that AMI decreases the amplitude of sIPSCs in young BF neurons. (F) Scatter plots with mean ± SEM of amplitude (left, control: 53.75 ± 9.32 pA, 3 μM AMI: 35.9 ± 4.58 pA, paired t-test, p = 0.07) and graph of normalized fold change in amplitude (right, 3 μM AMI: 0.78 ± 0.13, paired t-test, p = 0.11) show that AMI did not significantly alter the amplitude of sIPSCs in aged BF neurons. (G) Cumulative probability plots of sIPSC inter-event intervals in young mouse BF neurons (n = 10). (H) Cumulative probability plots of sIPSC inter-event intervals in aged mouse BF neurons (n = 9). In AMI, the cumulative probability curves were significantly different from control (*p < 0.01, K-S test). N = 5 young and N = 5 aged mice.