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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: Cell Microbiol. 2020 Dec 10;23(3):e13294. doi: 10.1111/cmi.13294

FIGURE 2. Localization of Pb22 protein by IFA.

FIGURE 2

Representative photomicrographs of fluorescent staining in WT parasites at different developmental stages. The parasites were incubated with anti-rPb22 (1:500) as the primary antibodies (green) without (left panel, -Triton X-100) or with (right panel, +Triton X-100) membrane permeabilization. The parasites were also labeled with antibodies against the markers for different stages (red) after membrane permeabilization. Makers include PbMSP1 for schizonts, P47 for female gametocytes and gametes, α-tubulin II (α-tub) for male gametocytes and gametes, SET for nuclei of gametocytes, and PSOP25 for ookinetes. Alexa Fluor 488 (AF488)-conjugated goat anti-mouse IgG antibodies and Alexa Fluor 555 (AF555)-conjugated goat-anti-rabbit IgG antibodies were used as the secondary antibodies. WT ookinetes labeled with anti-GST sera and with the secondary antibodies only were used as negative controls. Nucleus was stained with Hoechst-33258 (blue). Images were obtained under the same conditions at a magnification of 1000×. DIC, differential interference contrast microscopy. Scale bar = 5 μm.