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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: Cell Microbiol. 2020 Dec 10;23(3):e13294. doi: 10.1111/cmi.13294

FIGURE 4. Phenotypic analyses of pb22-KO and pb22-RE lines.

FIGURE 4

BALB/c mice were infected by intraperitoneal (i.p.) injection with 1×106 WT, pb22-KO (KO), or pb22-RE (RE) parasites. A. Exflagellation centers per field at 400× magnification. B. Macrogamete numbers in 0.01 μl of infected blood. C. Ookinete numbers in an in vitro culture assay (the same number of mature gametocytes among WT, KO and RE groups). D. Ookinete numbers in midguts 24 h post blood meal. Horizontal bars indicate the mean number (n = 10). E. Oocyst numbers per midgut in mosquitoes 12 days after feeding on WT, KO and RE parasites infected mice. Horizontal bars indicate the mean number (n = 30). D and E are representative graphs of experiment 1 in Table 1. Error bars indicate SD. **, P < 0.01. All experiments included three biological replicates.