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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: Cell Microbiol. 2020 Dec 10;23(3):e13294. doi: 10.1111/cmi.13294

FIGURE 7. In vivo transmission-blocking activity of rPb22 protein.

FIGURE 7

Six mice immunized with rPb22 and GST as well as the adjuvant control groups were infected with P. berghei parasites 10 days after the third immunization. A-C. Tail blood was collected at 3 days post-infection, and in vitro ookinete culture assay was used to obtain the macrogamete numbers in 0.01 μl of infected blood (A), exflagellation centers per field (B), and the ookinete numbers in 0.5 μl of culture (roughly equivalent to 0.01 μl of infected blood) (C). D. Representative graph of ookinete numbers in 10 midguts 24 h post blood meal. E. Representative graph of oocyst numbers per midgut 12 days post blood meal in all immunization groups. The D and E are representative graphs of experiment 1 in Table 2. Data are presented as mean ± SD. ** (P < 0.01) represents significance between GST-immunized and rPb22-immunized groups. The results are from three different biological replicates.