Table 1.
Ookinete numbers, oocyst density and prevalence in mosquitoes fed on WT, pb22–KO and pb22–restored (pb22–RE) parasites.
| Exp. | Parasite | Ookinete formation in midgut at 24 h | Oocyst formation at 12 days | |||
|---|---|---|---|---|---|---|
| Ookinete-positive midguts (no. dissected) | No. ookinetes/positive midgut [Mean (range)] | Infection prevalence (no. dissected) a | Reduction in prevalence b | Oocyst density [Mean (range)] c | ||
| 1 | WT | 9 (9) | 1187 (300–2730) | 93.3% (30) | 103.7 (0–300) | |
| pb22–KO | 0 (10) | 0 | 0 (30) | 93.3% | 0 | |
| pb22–RE | 10 (10) | 1154 (420–3000) | 93.3% (30) | 86.9 (0–302) | ||
| 2 | WT | 10 (10) | 2230 (500–4550) | 86.7% (30) | 100.7 (0–319) | |
| pb22–KO | 0 (10) | 0 | 0 (29) | 86.7% | 0 | |
| pb22–RE | 10 (10) | 2065 (750–5300) | 96.6% (29) | 97.5 (0–303) | ||
| 3 | WT | 10 (10) | 2280 (1000–3700) | 93.1% (29) | 110.6 (0–309) | |
| pb22–KO | 1 (10) | 5 (0–5) | 0 (28) | 93.1% | 0 | |
| pb22–RE | 9 (9) | 1985 (600–5050) | 86.7% (30) | 96.8 (0–302) | ||
a
The infection prevalence was calculated by the number of mosquitoes with oocysts / number of mosquitoes dissected in each group × 100%.
b
The percent reduction in oocyst prevalence was calculated as % mean prevalence wt – % mean prevalence Pb22-KO.
c
Mean number and range of oocysts per mosquito midgut.
Exp., Experiment.