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. 2021 May 28;9:673291. doi: 10.3389/fcell.2021.673291

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Copyright © 2021 Hu, Zhang, Wang, Yang, Qi, Zhang, Jiang, Zhang, Sun and Liang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of FAC on intracellular Fe²⁺ accumulation in porcine oocytes during IVM. (A) Representative images of the fluorescent probe FerroOrange showing intracellular Fe²⁺ levels in porcine oocytes. Scale bar = 100 μm. (B) Quantification of the relative intracellular Fe²⁺ levels in porcine oocytes from the different FAC treatment groups. The number of oocytes examined from each experimental group is indicated by the bars. Statistically significant differences are represented by different letters (p < 0.05).