Table 1.
Baseline characteristics of the subjects in study
| Discovery phase | Validation phase | |||||||
|---|---|---|---|---|---|---|---|---|
| HCs (n = 10) | OBI (n = 10) | P | HCs (n = 20) | OBI (n = 64)a | ASC (n = 20) | CHB (n = 31)b | P | |
| Demographic characteristics | ||||||||
| Female (n, %) | 1 (10.00) | 2 (20.00) | 0.53 | 6 (30.00) | 22 (34.38) | 7 (35.00) | 10 (41.94) | 0.98 |
| Age (years) | 39.30 ± 11.68 | 42.80 ± 7.96 | 0.45 | 38.00 ± 7.94 | 42.86 ± 8.81 | 36.00 ± 7.48 | 43.11 ± 14.16 | 0.09 |
| Serological characteristics | ||||||||
| ALT (U/L) | 19.56 ± 7.63 | 22.70.67 ± 11.14 | 0.48 | 16.74 ± 6.41 | 18.99 ± 9.79 | 37.80 ± 8.23 | 219.94 ± 263.70 | 0.13 |
| HBsAg (S/CO, positive) |
0.31 ± 0.30 0 |
0.12 ± 0.16 0 |
0.12 |
0.21 ± 0.25 0 |
0.16 ± 0.21 0 |
22.89 ± 9.91 20 |
272.28 ± 139.62 31 |
< 0.01c |
| Anti-HBs (S/CO, positive) |
7.00 ± 8.32 5 |
0.87 ± 0.72 3 |
0.04 |
8.24 ± 8.18 12 |
0.77 ± 1.21 7 |
0.21 ± 0.24 0 |
0.23 ± 0.72 0 |
< 0.01c |
| HBeAg (S/CO, positive) |
0.08 ± 0.03 0 |
0.07 ± 0.02 0 |
0.89 |
0.07 ± 0.01 0 |
0.05 ± 0.02 0 |
0.06 ± 0.01 0 |
38.91 ± 82.97 7 |
0.46c |
| Anti-HBe (S/CO, positive) |
1.60 ± 0.31 0 |
1.28 ± 0.47 2 |
0.11 |
1.88 ± 0.12 0 |
0.97 ± 0.40 8 |
0.24 ± 0.34 19 |
8.53 ± 30.90 24 |
< 0.01c |
| Anti-HBc (S/CO, positive) |
1.48 ± 0.47 1 |
0.71 ± 0.73 7 |
0.02 |
1.96 ± 0.40 0 |
0.45 ± 0.53 28 |
0.08 ± 0.07 20 |
11.03 ± 1.84 31 |
< 0.01c |
| HBV-DNA (Ct) | NA | 34.89 ± 1.25 | NA | NA | 35.86 ± 1.87 | 33.67 ± 3.01 | 23 ~ 2.88E + 8 | < 0.01c |
OBI occult HBV infection, CHB chronic hepatitis B, ASC asymptomatic HBsAg carriers, HC healthy controls, HBsAg hepatitis B surface antigen, Anti-HBs antibody for HBsAg, HBeAg hepatitis B envelope antigen, Anti-HBe antibody for HBeAg, Anti-HBc antibody for hepatitis B core antigen, ALT alanine aminotransferase, Ct cycle threshold, NA not available
aDue to limited volume, the results of Anti-HBs, HBeAg, Anti-HBe and Anti-HBc were only available for 34 OBI samples
bHBsAg, Anti-HBs, HBeAg, Anti-HBe and Anti-HBc were measured with Anytest-2000 TRF detection system, and HBV DNA was detected with HBV DNA PCR-fluorescence quantitative diagnostic kit(KHB)
cP value indicated the statistic differences between HCs, OBI and ASC groups