Skip to main content
. 2021 May 28;9:607001. doi: 10.3389/fcell.2021.607001

FIGURE 7.

FIGURE 7

SOCS3 overexpression inhibited the FAK/Src pathway and reversed the promotion of invasion induced by BMSCs that differentiated into fibroblasts after the action of EVs. (A,B) SOCS3 levels were detected by RT-qPCR and Western blot analysis to verify the transfection efficiency. (C) α-SMA and vimentin in BMSCs were detected by immunofluorescence. (D) levels of α-SMA, vimentin and the FAK/Src pathway-related proteins in hepatoma cells were detected by Western blot analysis. (E) MTT assay was used to detect the proliferation ability of Huh7 and H22 cells at different time points. (F) EdU assay was used to detect the proliferation ability of Huh7 and H22 cells. (G,H) Transwell assay was used to detect the invasion and migration ability of Huh7 and H22 cells. **p < 0.01, ***p < 0.001. Data in panels (A,B) were analyzed by the t test, and data in panels (D–H) were analyzed by one-way ANOVA, followed by Tukey’s multiple comparison test. Replicates = 3.