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ROF inhibits IL-1β–induced inflammatory responses and cartilage degradation in chondrocytes. Cells were treated with different concentrations of ROF in the presence or absence of IL-1β (10 ng/ml) for 24 h. (A) Western blots and (B) quantitative analysis of iNOS and COX-2. (C) Western blots and (D) quantitative analysis of collagen Ⅱ, MMP13, and ADAMTS5. GAPDH was employed as the loading control (n = 3). #p < 0.05 vs. control group; *p < 0.05 and **p < 0.01 vs. IL-1β group.
