Figure 5.

Expression of Δ133p53α in murine T cells is associated with reduced TIGIT levels and improved T-cell effector function. (A) Representative, flow cytometric data of TIGIT and PD-1 expression of mouse T cells obtained from CyA2K^b mice, transduced with scTCR and of T cells obtained from OT-I mice (B). Both were transduced with human Δ133p53α or empty vector. Quantitative data from a representative tumor colony-forming assay using CyA2K^b TCR mouse T cells and A2K^bMEF^mutp53 (C). For OT-I cells, MC38^OVA and B16^OVA served as target cells (D). The optical density (O.D.) indicates the amount of remaining viable tumor colonies after incubation with effector T cells. PD-1, programmed cell death 1; TCR, T cell-receptor.