(A) Proposed SAXS model 3 (SM-3) of the KRas4B1-185-CaM complex. SM-3 was aligned within the calculated envelope of FME KRas4B1-185-GppNHp SAXS data. The hybrid model of full-length KRas4B1-185 (dark blue) and CaM (light blue) is shown in the SAXS envelope.
(B) The experimental SAXS data (open circles) is plotted with theoretical scattering of SM-3 (green, χ2 = 2.12), and a weighed theoretical scattering of SM-3 (25%) and SM-2 (75%) (pink, χ2 = 1.65). The theoretical scattering of SM-2 (blue) is also plotted for comparison. Weighing calculations were performed in MultiFoXS (Schneidman-Duhovny et al., 2016).